仪器列表
分析仪器 281
计量仪器 7
大气探测仪器 0
实时活细胞分析系统(Incucyte SX5)二号板虚拟
实时活细胞分析系统(Incucyte SX5)二号板虚拟
仪器编号
HTCF-AW005-2
规格
生产厂家
Sartorius
型号
Ultimate Lite/A11613
制造国家
美国
分类号
011099
放置地点
E8-B118-b
出厂日期
2023-04-13
购置日期
2023-04-13
入网日期
2023-08-18

主要规格及技术指标

硬件: 4倍、10倍和20倍高清物镜,可自动切换;高清相差成像;绿色/红色荧光模块:绿色荧光通道:激发波长441-481nm,发射波长503-544nm;红色荧光通道:激发波长567-607nm,发射波长:622-704nm;绿色/橙色/近红外光学模块:绿色荧光通道:激发波长453-485nm,发射波长494-533nm;橙色荧光通道:激发波长546-568nm,发射波长576-639nm;近红外荧光通道:激发波长648-674nm,发射波长685-756nm; 板位:具备6个板位,可放置6、24、48、96孔板;具有全孔成像功能,能够全视野观察细胞; 动态拍照扫描间隔可调20min-12h 软件:包含细胞增殖、凋亡、毒性、细胞周期等常规分析功能;免疫细胞杀伤(ADCC);报告基因检测/转染效率检测;免疫细胞成簇/聚集;中性粒细胞外捕网检测;细胞单克隆;抗体内化;细胞吞噬;活细胞免疫荧光;细胞趋化分析(对微孔板中无标记和荧光标记的趋化性细胞进行迁移和侵袭的追踪和定量分析);肿瘤球分析(分析圆底多孔板中单肿瘤球的生长、活力或侵袭,或者检测平底孔板中的多肿瘤球的生长或活力变化);类器官分析(表征在 24 或 48 孔板中培养的类器官的分化和成熟,在 96 孔板中评估处理对类器官生长的影响);无标记神经生长分析(可在 96 或 384 孔板的每个孔中进行神经突生长的无标记或荧光分析); Hardware :
4x, 10x, and 20x objectives on an automated turret;
HD Phase imaging mode;
Green/Red Optical Module:Green Fluorescence channels: EX:441-481nm,EM: 503-544nm; Red Fluorescence channels: EX:441-481nm,EM: 503-544nm;
Green/Orange/NIR Triple Color Optical Module:Green Fluorescence channels: EX:453-485nm,EM: 494-533nm; Orange Fluorescence channels: EX:546-568nm,EM: 576-639nm; NIR Fluorescence channels:EX 648-674nm,EM685-756nm
Six plates for multi-well plates;
The scanning interval can be adjusted from 30min-12h;
Imaging & Analysis Software:
Proliferation/Apoptosis/Toxicity/Cell cycle;Immune cell killing;Reporter gene detection/Transfection efficiency detection;Immune cell cluster;Netosis Assay;Cell monoclonal;Antibody internalization;Cell phagocytosis;Live cell immunofluorescence;Chemotaxis(Analyze label-free and fluorescently labeled chemotactic cell migration or invasion while qualitatively monitoring cell morphology of every cell within each well of a Incucyte® 96-well Clearview Plates.);3D Tumor Spheroid(can be used to quantitate invasive growth and shrinkage in single spheroid models grown in 96-well round-bottom ULA plates) ; Organoid Analysis(enables automated acquisition and label-free analysis of organoids embedded in Matrigel.Characterize the differentiation and maturation of organoid cultures in 24- or 48- well plates and assess treatment effects on organoid growth in 96-well microplates);NeuroTrack(analyze neurite dynamics with or without labels in living cells in each well)

主要功能及特色

可在培养箱内进行长时程实时动态观测细胞生长过程。拍摄过程中载物台不动,通过自动移动物镜转盘实现多个孔板的拍摄。自动对焦,对每个时间点、每块板、每个孔执行自动对焦,并自动采集活细胞随时间变化的图像,根据时间变化的图像可以输出动态录像。明场状态下能够分析细胞密度、细胞面积、细胞团数量等指标;还可自动统计荧光细胞数量、荧光密度、每个细胞荧光强度、荧光面积等指标。可以直接从软件中抓取图像、图表、曲线至Word、Excel、Powerpoint和Email中,可自动生成浓度反应曲线,计算EC50或IC50值。 Analyze cells for days, weeks or even months as they sit stationary in the stable environment of incubator.Scan by the objectives on an automated turret.Autofocus is performed on each time point, plate, and well, and images of living cells over time are automatically acquired.
Cell density, cell area, number of cell masses and other indicators can be analyzed by phase contrast images .
Automatically count the number of fluorescent cells, fluorescence density, fluorescence intensity of each cell, fluorescence area and other indicators.Images, charts, and curves can be directly captured from the software to Word, Excel, Powerpoint, and Email, and concentration response curves can be automatically generated to calculate EC50 or IC50 values.

主要附件及配置

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