Micro-beta2微孔板液闪计数器是使用液体闪烁体(闪烁液)接受射线并转换成荧光光子的放射性计量仪。这两款仪器具有16个样品架容量,包括2个探头。计 数 模 式 包 括 单 标 记 、双 标 记 C P M,单 标 记 、双 标 记DPM,ParaLuxTM计数模式和化学发光计数。与150μL闪烁液混合的未淬灭样品,滴入96孔的微孔板:计数效率: 3H 通常为 57 %,14C 通常为 94 %最大计数率: 3,000,000 CPM。自集β计数、γ计数和发光测定为一体,主要利用3H、14C、32P、35S、125I等放射性核素进行体内或体外标记,研究细胞生物体内核酸、蛋白质等生物分子的合成与降解代谢及其转化途径。MicroBeta®2 is microplate counters that measure radioactivity, as well as luminescence.1. 2-detector systems run 24- and 96-well plates. 2. Counting modes include single- and dual-label CPM, single- and dual-label DPM, ParaLux™ count mode, and luminescence counting.3. PMT positioning for the measurement is automatic. Maximum height of the sample for 16-shelf MicroBeta2 is 45 mm, or 20 mm with robotic loading shelf. 4.Unquenched sample with a volume of 150 μL of cocktail unpurged, in a flexible 96-well microtiter plate:
Counting efficiency: 3H Typically 57 %, 14C Typically 94 %
Maximum count rate: 3,000,000 CPM
1. 固相均相检测:所有固体均相检测中均可提供一流的检测性能;
2. 细胞增殖检测:通过基于滤光片的H3标记的胸腺嘧啶吸收量分析细胞增殖的完美选择;
3. 细胞毒性检测:采用Lumaplate技术或液体闪烁计数(LSC)进行51Cr释放检测,以分析细胞毒性;
4. 报告基因检测:用于采用荧光素酶和β内酰胺酶进行双重报告基因检测的慢速和快速化学发光检测功能;
5. 代谢研究:使用Lumaplate技术或传统的混合物方法在4ml管中进行HPLC馏分测定;
6. 液体闪烁计数:将传统的样品瓶液体闪烁计数检测缩小到微孔板上;
7. 擦拭试验:需要准备另一台计数仪用于检测实验室的污染情况;
8. 更多选择:发光ELISA,激酶检测,放射性免疫测定(RIA)以及更多其他分析。
8.1.Advanced detectors - capable of detecting β and γ isotopes, as well as glow-type chemiluminescence and flash-type chemiluminescence
8.2.Filter-based receptor binding analysis - Use UniFilter well plates or MicroBeta Fly to provide the highest sensitivity
8.3.Solid-Phase Homogeneity Experiments - Best-in-class detection performance in all solid scintillation assays, including bead-based scintillation approximation experiments and FlashPlate experiments
8.4.Cell proliferation - Analyze cell proliferation by membrane-based 3H-labeled thymidine uptake
8.5.Cytotoxicity – Cytotoxic 51Cr release assays using LumaPlate or liquid scintillation counting (LSC).
8.6.Reporter Assay - Glow-type chemiluminescence and flash-type chemiluminescence for dual reporter detection using luciferase and β-lactamase
8.7.Metabolic studies - HPLC segmentation measurements in 4 mL vials using LumaPlate or traditional scintillation solution-based methods
8.8.Liquid scintillation counting - Shrinks traditional vial-based liquid scintillation counting analysis to microplates
8.9.Wipe test - no additional counter is required to monitor contamination in the laboratory
8.10.More options - ELISA, kinase experiment, radioimmunoassay (RIA), etc"
96孔板、24孔板、4 ml样品瓶、闪烁液
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