大型仪器共享管理系统

"2.1 全自动操作：单细胞水平的显微注射，可以对贴壁细胞进行注射；单细胞提取，可以对单细胞进行提取；单细胞分离，可以对细胞或者beads进行分离；单细胞力谱，可以测量单细胞力谱。全过程通过软件设置、点击鼠标即可完成；
*2.2 注射速度：不小于100个细胞每小时；
*2.3 注射精度：不大于1pL；
2.4 可进行细胞核、细胞质精准定位注射；
*2.5 探针更换方式：软件自动更换，无需手动加载；
2.6 样品台适配：支持标准6、12、24孔板，5 cm培养皿、载玻片；；
2.7 样品台移动方向：X、Y、Z轴均可移动；
*2.8 样品台移动精度：双向XY全量程重复性< 500nm，双向Z重复性<5 nm；
2.9 样品台移动范围：XY轴不小于200 x 70 mm ；Z轴不小于3cm；
2.10 配置激光引导力学感应头，具备力学探测反馈功能；
2.11 探针力学控制：提供探针当前压力值并绘制曲线；
2.12 软件系统：用鼠标点击屏幕上的细胞，由系统自行完成注射、提取、分离、单细胞力谱；软件功能：可同时采集分析处理数据，实时输出微流控数据与力学信号；
2.13 防震模块：负载：≥150 kg；功率：207W；重量：272 kg；
2.14 工作站：处理器：Intel Core i7，操作系统：Windows系统；显卡：HD4600，内存：≥8GB，硬盘不低于：1TB，高清触摸屏显示器：27英寸；接口类型：USB/HDMI。
2.15 微流控模块：
2.15.1 配置高灵敏度液体微流控系统，压力范围：-800mba 到1000mbar；
2.15.2 流体压力控制：压力大小、时间，并自动绘制输出压力曲线；
2.15.3 流体压力控制精度： 1 mbar；
2.16 荧光成像模块
2.16.1 配置倒置显微镜，具有荧光成像功能；
2.16.2 至少配置10X、20X、UCPLFLN20X各一个，可观测RFP、GFP、DAPI，单色CMOS；
2.16.3 电动六孔物镜转盘；
2.17 活细胞培养模块
2.17.1 具有紫外灯、LED照明装置；
2.17.2 具有Hepa过滤装置、恒温控制单元、CO2控制装置，适用于长时间的细胞培养；
2.17.3 CO2浓度范围：1%至15%可调节。
2.1 Automatic operation: Microinjection at the single-cell level, which can inject adherent cells; Single cell extraction, can be extracted from a single cell; Single cell separation, cells or beads can be separated; Single cell force spectrum. You can measure single cell force spectrum. The whole process can be completed by software setting and clicking the mouse.
*2.2 Injection rate: not less than 100 cells per hour;
*2.3 Injection accuracy: no more than 1pL;
2.4 Accurate localization injection of nucleus and cytoplasm;
*2.5 Probe replacement method: software automatic replacement, without manual loading;
2.6 Sample table adaptation: support standard 6, 12, 24 well plates, 5 cm petri dishes and slides;
2.7 Moving direction of the sample platform: X, Y and Z axes can be moved;
*2.8 Sample table movement accuracy: bidirectional XY full range repeatability < 500nm, bidirectional Z repeatability < 5nm;
2.9 Moving range of sample platform: XY axis not less than 200 x 70 mm; The z-axis is not less than 3cm;
2.10 Equipped with laser guided mechanical induction head, equipped with mechanical detection and feedback function;
2.11 Mechanical control of the probe: provide the current pressure value of the probe and draw a curve;
2.12 Software system: Click the cell on the screen with the mouse, and the system will complete the injection, extraction, separation and single-cell force spectrum by itself; Software functions: can simultaneously collect, analyze and process data, real-time output microfluidic data and mechanical signals;
2.13 Shockproof module: Load: ≥150 kg; Power: 207W; Weight: 272 kg;
2.14 Workstation: Processor: Intel Core I7, operating system: Windows; Graphics card: HD4600, memory: ≥8GB, hard disk: no less than 1TB, HD touch screen display: 27 inches; Port type: USB/HDMI.
2.15 Microfluidic module:
2.15.1 Equipped with highly sensitive liquid microfluidic system, pressure range: -800MBA to 1000mbar;
2.15.2 Fluid pressure control: pressure size and time, and automatically draw the output pressure curve;
2.15.3 Fluid pressure control accuracy: 1 mbar;
2.16 Fluorescence imaging module
2.16.1 Inverted microscope with fluorescence imaging function;
2.16.2 Configure at least one 10X, 20X, and UCPLFLN20X, which can observe RFP, GFP, DAPI, and monochrome CMOS;
2.16.3 Electric six-hole objective turntable;
2.17 Live cell culture module
2.17.1 Equipped with UV lamp and LED lighting device;
2.17.2 Equipped with Hepa filter device, constant temperature control unit and CO2 control device, suitable for long-term cell culture;
2.17.3 CO2 concentration range: adjustable from 1% to 15%."

"FluidFM技术是国际最前沿的技术，在单细胞组学和活细胞单细胞测序领域、在单细胞CRISPR基因编辑、单细胞提取、单细胞分离分选领域、在细胞骨架力学研究领域、在单细胞相互作用研究领域都具有无可争议的领先地位。这款精度高、自动化程度高的单细胞分辨率水平的科研工具正是我们迫切需要引进的设备。多功能单细胞显微操作系统可应用于干细胞领域、基因工程、表观遗传学、药物开发、毒理学、干细胞、癌细胞研究、细胞系开发等，应用前景广泛，是目前科研教学必备的仪器设备；除此以外，多功能单细胞显微操作系统还能够胜任点打印等。
FluidFM technology is the most cutting-edge technology in the world. It has an undisputed leading position in the field of single cell omics and living cell single cell sequencing, single cell CRISPR gene editing, single cell extraction, single cell separation and sorting, cytoskeleton mechanics research and single cell interaction research. This scientific research tool with high precision and high degree of automation at single cell resolution level is exactly the equipment we urgently need to introduce. Multifunctional single-cell microoperation system can be applied in stem cell field, genetic engineering, epigenetics, drug development, toxicology, stem cell, cancer cell research, cell line development, etc., has a wide application prospect, and is an essential instrument for scientific research and teaching. In addition, multifunctional single-cell microoperating systems are capable of point-printing and other tasks,"

主要规格及技术指标

主要功能及特色

主要附件及配置