大型仪器共享管理系统

"1.主要性能
★1.1检测通道数：3通道以上
★1.2升降温速度不小于5℃/Sec
1.3加热冷却方式：半导体或Peltier
1.4温度范围：0–100˚C
1.5温控精确度：±0.2℃
★1.6必须有梯度PCR功能，梯度范围：1~24℃；温度范围：30–100˚C
1.7激发光光源：LED
1.8检测系统：检测灵敏度：能检测人类基因组中的单拷贝1b-actin基因，
1.9动态范围：10个数量级；
1.10检测器：PDT或PMT
1.11试剂反应体积：可做低反应体系≤5ul
1.12试剂、耗材：完全开放，各种科研和临床试剂适用；可采用0.2ml PCR单管，8联管、12联管、96孔PCR板，PCR联管；无需适配器
★1.13仪器无需校正
1.14实验样品通量：每次能进行96×0.2ml的样品检测，
1.15可同时进行2种以上种荧光信号的检测，具有实时分析数据的功能
★2.软件：
除程序设置、标准曲线定量、熔点曲线分析、等位基因分型外，还可进行基因表达分析，自动归一化，根据表达差异绘图等；可同时进行 5000个样品数据分析，有终点法分析功能。
1、支持Windows及Mac操作系统（注意MAC版本是要收费的）
2、支持柱形图、箱型图、点图、散点图、火山图、聚类图
3、支持ANOVA方差分析功能与t-检验等统计学功能
4、自定义报告导出
5、支持多内参基因选择功能
6、符合21CFR Part 11规定
1. Main performance★1.1 number of detection channels: more than 3 channels★1.2 l cooling speed is not less than 5℃/Sec1.3 heating and cooling: semiconductor or Peltier1.4 temperature range: 0 -- 100 serving C1.5 temperature control accuracy: ±0.2℃★1.6 must have gradient PCR function, gradient range: 1~24℃; Temperature: 30 to 100 degrees Celsius1.7 excitation light source: LED1.8 detection system: detection sensitivity: it can detect the single copy of 1b-actin gene in the human genome.1.9 dynamic range: 10 orders of magnitude;1.10 detector: PDT or PMTReagent reaction volume: low reaction system ≤5ul1.12 reagent and consumables: completely open, suitable for various scientific research and clinical reagents; Single tube, 8-tube, 12-tube, 96-well PCR plate and PCR tube can be used for 0.2ml PCR. No adapter requiredNo calibration is required for the instrument1.14 flux of experimental samples: 96×0.2ml samples can be tested each time.1.15 more than two kinds of fluorescence signals can be detected simultaneously, with the function of real-time data analysisU 2. Software:In addition to program setting, quantitative analysis of standard curve, melting point curve analysis and allele typing, gene expression analysis can also be carried out, automatic normalization and mapping based on expression differences. It can analyze the data of 5000 samples at the same time and has the function of endpoint analysis.1. Support Windows and Mac operating systems (note that the Mac version will be charged)2. Support column diagram, box diagram, point diagram, scatter diagram, volcano diagram and cluster diagram3. Support statistical functions such as ANOVA and t-test4. Custom report export5. Multiple internal reference gene selection function is supported6. It conforms to the provisions of 21CFR Part 11"

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荧光定量PCR是通过荧光染料或荧光标记的特异性的探针，对PCR产物进行标记跟踪，实时在线监控反应过程，结合相应的软件可以对产物进行分析，计算待测样品模板的初始浓度。具有特异性好，灵敏度高，准确性高，检测范围宽，操作简单、安全、无污染，速度快、通量高的特点。
Fluorescence quantitative PCR is to label and track the PCR products through the specific probe of fluorescent dye or fluorescent labeling, and monitor the reaction process online in real time. Combining with the corresponding software, the products can be analyzed to calculate the initial concentration of the sample template to be tested. It has the characteristics of good specificity, high sensitivity, high accuracy, wide detection range, simple operation, safety, no pollution, fast speed and high flux."

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