"1、主要技术规格:
1.1、 样品容量:384孔PCR板
★1.2、激发光源为: 5个不同波长LED,使用寿命为≥105小时。
1.3、样品体积:1 - 30µl
1.4 、升降温速度:2.5℃/秒
1.5、温度范围:0-100℃
1.6、温度准确性:± 0.2°C
★1.7、具有温度梯度功能; 梯度范围:30–100°C,梯度温度差范围:1℃ - 24℃
1.8、线性范围:10个数量级
★1.9、多重检测能力:4个基因/每孔
1.10、具有FRET检测功能
1.11、经过运输或搬动不需要校正就可检测
★1.12、8.5英寸彩色LCD触摸屏,可通过仪器面板设置运行程序。
1.13、配正版软件:具备多种分析模式:基于标准曲线的绝对定量;相对标准曲线;基于比较Ct值的相对定量;融解曲线分析;阴性/阳性结果判定;基因分型等。
1.14、软件可自由安装于多台电脑,方便操作分析
2、软件:
除程序设置、标准曲线定量、熔点曲线分析、等位基因分型外,还可进行基因表达分析,自动归一化,根据表达差异绘图等;可同时进行 5000个样品数据分析,有终点法分析功能。
2.1、支持Windows及Mac操作系统(注意MAC版本是要收费的)
2.2、支持柱形图、箱型图、点图、散点图、火山图、聚类图
2.3、支持ANOVA方差分析功能与t-检验等统计学功能
2.4、自定义报告导出
2.5、支持多内参基因选择功能
2.6、符合21CFR Part 11规定
1. Main technical specifications:1.1 sample capacity: 384 Wells PCR plate★1.2. The excitation light source is: LED with 5 different wavelengths, and the service life is ≥105 hours.1.3 sample volume: 1-30 l1.4 rise/drop speed: 2.5℃/ second1.5. Temperature range: 0-100℃1.6. Temperature accuracy: ± 0.2°C★1.7 with temperature gradient function; Gradient range: 30-100 °C, gradient temperature difference: 1 ° c-24 °C1.8. Linear range: 10 orders of magnitude★1.9. Multiple detection capability: 4 genes/hole1.10 have FRET detection function1.11. It can be detected without correction after transportation or moving★1.12, 8.5 inch color LCD touch screen, can be set through the instrument panel to run the program.1.13. With legitimate software: multiple analysis modes: absolute quantification based on standard curves; Relative standard curve; Based on the relative quantitative comparison of Ct values; Fusion curve analysis; Determination of negative/positive results; Genotyping and so on.1.14. The software can be freely installed on multiple computers for easy operation and analysis2. Software:In addition to program setting, quantitative analysis of standard curve, melting point curve analysis and allele typing, gene expression analysis can also be carried out, automatic normalization and mapping based on expression differences. It can analyze the data of 5000 samples at the same time and has the function of endpoint analysis.2.1 support for Windows and Mac operating systems (note that the Mac version will be charged)2.2 support column diagram, box diagram, point diagram, scatter diagram, volcano diagram and cluster diagram2.3 statistical functions such as ANOVA and t-test are supported2.4. Custom report export2.5. Multiple internal reference gene selection function is supported2.6 comply with 21CFR Part 11
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荧光定量PCR是通过荧光染料或荧光标记的特异性的探针,对PCR产物进行标记跟踪,实时在线监控反应过程,结合相应的软件可以对产物进行分析,计算待测样品模板的初始浓度。具有特异性好,灵敏度高,准确性高,检测范围宽,操作简单、安全、无污染,速度快、通量高的特点。
Fluorescence quantitative PCR is to label and track the PCR products through the specific probe of fluorescent dye or fluorescent labeling, and monitor the reaction process online in real time. Combining with the corresponding software, the products can be analyzed to calculate the initial concentration of the sample template to be tested. It has the characteristics of good specificity, high sensitivity, high accuracy, wide detection range, simple operation, safety, no pollution, fast speed and high flux."
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