主要规格及技术指标

"1. 基本指标
1.1.检测模式：包含光栅式光吸收及高速扫描、荧光顶部底部、TRF、FRET、TR-FRET、荧光扫描（3D）、荧光偏正（FP）、发光（闪光和辉光）、高灵敏发光扫描、多色发光（BRET123，Chroma-Glo）、Alpha技术（Alphalisa，Alphaplex）；
1.2.读板方法：终点、动力学、光谱扫描、孔区域扫描；
1.3.微孔板类型：可适用于1 - 1536孔板，微量检测板, 比色皿；
1.4. 检测器3个：光吸、收荧、光化学发光均使用独立检测器：
1.4.1.光吸收检测器: 光电二极管;
1.4.2．荧光检测器: 红外敏感光电倍增管（PMT）;
1.4.3发光检测器: 低暗电流单光子计数光电倍增管；
1.5.具备振荡功能：线性、圆形、双轨模式可选且振幅和频率可调；
1.6.温度控制范围：室温+3℃~42℃,温度控制精度：±0.5°C ；
1.7.最快读板时间：全波长扫描时间≤5s；
2. 光吸收模块
2.1光栅：光吸收高速光栅完成200-1000nm,扫描时间＜5sec/孔；
2.2. 光吸收波长准确性:≤±0.3nm；
2.3. 光吸收波长重复性:≤±0.3nm；
2.4.带宽：3.5nm；
2.5. OD 精确度：≤ 0.2 %，OD准确度: ≤0.5%;

3.荧光模块
3.1. 荧光波长范围: Ex：230–900nm, Em：280–900nm，1nm步进连续可调;
3.2.内置滤光片/四光栅结合的融合光路设计，具有四种检测模式，分别为光栅(M)/光栅(M)，光栅(M)/滤光片(F)，滤光片(F)/光栅(M)，滤光片(F)/滤光片(F)满足客户不同的实验需求；
3.3.荧光滤光片系统灵活，激发滤光片卡槽与发射滤光片卡槽相互独立，共有6×6种滤光片组合方式。
3.4.荧光顶部阅读灵敏度:
检测限F/F-≤25 amol/well 荧光素，
检测限M/M：≤50amol/well荧光素；
3.5.具有荧光底部多点读数功能，通过覆盖全孔的多点读数，降低细胞分布不均匀带来的孔间和孔内检测差异；
3.5.同时具有顶部，底部2种Z-轴自动调焦功能，14800-23000μm连续可调；

4.时间分辨荧光模块
4.1.波长范围Ex: 230 – 900 nm; Em: 280 – 900 nm，
4.2.灵敏度: 检测限F/F ≤4 amol/孔 铕；
检测限M/M ≤10 amol/孔 铕；

5.荧光偏振模块
5.1.荧光偏振波长范围300-800nm，
5.2.检测限检测限F/F≤ 1.25 mP （1nm荧光素），

6. 化学发光模块
6.1.灵敏度：连续发光 225 amol ATP/孔 (9 pM; low volume 384孔板)；
瞬时发光: 12 amol ATP/孔 (218 fM; 384孔板);
6.2. 化学发光检测线性范围：> 8个数量级

7. Alpha Screen检测模块：
7.1．光源：独立激光光源
7.2.支持检测模式：AlphaScreen，AlphaLISA，AlphaPlex
7.3.具有校准孔间温度差的红外传感器，具有温度稳定功能，具有温度校准功能更佳
7.3. 检测限≤100 amol/well bio-LCK-P;20μL

8.加样器模块
8.1.支持板型：1 – 384孔板
8.2.注射泵容积：0.5 ml，1 ml，2.5ml可选
8.3.加样体积：5 - 2500 µl/次; 1 µl步进
8.4.死体积：≤ 100 µl

9.自动开盖模块
9.1. 内置Lid Lifter模块，可在读板前自动开盖，读板后自动加盖，防止样品被污染和溶剂挥发对实验造成影响；

10.湿度控制模块
10.1.具有湿度控制模块，较少长时间检测样品边缘效应；
11、细胞计数
尺寸范围：4–90µm
计数精度：+/-10%（10–30µm）
计数再现性<10%（10–30µm）
细胞浓度：1x104–1x107细胞/毫升
样本数/最多运行8个样本

12、自动细胞成像
照明：大功率LED
目标：4x
光学分辨率：1.3µm/像素
读取速度：图像/孔（96孔板）；<5分钟
13 .气体控制模块
可调浓度范围–CO2 0.04–10%（体积）
可调浓度范围–O2 0.1–21%（体积）
浓度精度–CO2<1%（体积）
浓度精度–O2<0.5%（体积）

14、温度控制
环境温度+3°C至42°C
15.Te-冷却模块
温度范围+18–42°C
冷却功率: 最高温度低于环境温度12°C
1. Technical parameters:
1. Basic Indicators
1.1. Detection mode: including raster light absorption and high-speed scanning, fluorescence top and bottom, TRF, FRET, TR-FRET, fluorescence scanning (3D), fluorescence polarization (FP), luminescence (flash and glow), highly sensitive luminescence Scanning, multicolor luminescence (BRET123, Chroma-Glo), Alpha technology (Alphalisa, Alphaplex);
1.2. Plate reading methods: endpoint, kinetics, spectral scanning, well area scanning;
1.3. Microplate type: suitable for 1 - 1536 well plates, micro-detection plates, cuvettes;
1.4. 3 detectors: independent detectors are used for light absorption, fluorescence absorption, and photochemiluminescence:
1.4.1. Optical absorption detector: photodiode;
1.4.2. Fluorescence detector: infrared sensitive photomultiplier tube (PMT);
1.4.3 Luminescence detector: low dark current single photon counting photomultiplier tube;
1.5. With oscillation function: linear, circular, dual-track modes are optional, and the amplitude and frequency are adjustable;
1.6. Temperature control range: room temperature +3°C~42°C, temperature control accuracy: ±0.5°C;
1.7. The fastest plate reading time: Scan speed (200–1,000 nm) ≤ 5s;

2. Light Absorption Module
2.1 Spectral range: 200-1000nm, scanning time <5sec/hole;
2.2. Wavelength accuracy: ≤±0.3nm;
2.3. Wavelength reproducibility : ≤±0.3nm;
2.4. Bandwidth: 3.5nm;
2.5. OD Precision: ≤ 0.2 %, OD accuracy: ≤ 0.5%;

3. Fluorescence Module
3.1. Fluorescence wavelength range: Ex: 230–900nm, Em: 280–900nm;
3.2. Integrated optical path design with built-in filter/four gratings, with four detection modes, grating (M)/grating (M), grating (M)/filter (F), filter (F) )/grating (M), filter (F)/filter (F) to meet the different experimental needs of customers;
3.3. The fluorescence filter system is flexible. The excitation filter slot and the emission filter slot are independent of each other. There are 6×6 filter combinations.
3.4. Fluorescence intensity:
Filter - top ≤25 amol/well (100 µl; 384 well)
Mono - top ≤50 amol/well (100 µl; 384 well);
3.5. It has the function of multi-point reading at the bottom of the fluorescence. Through the multi-point reading covering the whole well, the detection difference between wells and within wells caused by uneven cell distribution is reduced;
3.5. At the same time, it has two types of Z-axis auto-focusing functions at the top and bottom, which are continuously adjustable from 14800-23000μm;

4. Time-resolved fluorescence module
4.1. Wavelength range Ex: 230 – 900 nm; Em: 280 – 900 nm,
4.2Limit of detection Filter ≤4 amol/well (100 µl; 384 well);
Limit of detection Mono≤10 amol/well (100 µl; 384 well);

5. Fluorescence Polarization Module
5.1. Wavelength range 300-800nm,
5.2. Precision Filter ≤ 1.25 mP ,

6. Luminescence module
6.1.Luminescence (glow):
Limit of detection≤ 225 amol/well (25 µl; 384 sv well);
Luminescence (flfl ash) :
Limit of detection≤ 12 amol/well (55 µl; 384 well);
6.2.Dynamic range > 9 orders of magnitude

7. AlphaScreen module:
7.1. Light source: independent laser light source
7.2. Support detection modes: AlphaScreen, AlphaLISA, AlphaPlex
7.3. Infrared sensor with calibrated temperature difference between holes, with temperature stabilization function, better with temperature calibration function
7.3. Limit of detection
< 100 amol/well bio-LCK-P6; 20 µl

8. Sampler module
8.1. Supported plate type: 1 – 384 well plate
8.2. Syringe pump volume: 0.5ml, 1ml, 2.5ml optional
8.3. Injection volume: 5 - 2500 µl/time; 1 µl step
8.4. Dead volume: ≤ 100 µl

9. Automatic cover opening module
9.1. Built-in Lid Lifter module, which can automatically open the cover before reading the plate, and automatically add the cover after reading the plate, to prevent the sample from being contaminated and the solvent volatilization affecting the experiment;

10. Humidity control module
10.1. With a humidity control module, the edge effect of the sample is less detected for a long time;

11. Cell counting
Size range: 4–90 µm
Counting accuracy : +/–10% (10–30 µm)
Counting reproducibility < 10% (10–30 µm)
Cell concentration: 1x104–1x107 cells/ml
Imaging speed inc. data reduction < 30 sec/sample
Number of samples/run up to 8 samples

12.Automated cell imaging
Illumination: High power LED
Image: Bright-field
Objective: 4x
Autofocus: Laser based
Optical resolution :1.3 µm/pixel
Read Speed: image/well (96-well plate); < 5min

13 .Gas Control Module
Adjustable concentration range – CO2 0.04–10% (vol.)
Adjustable concentration range – O2 0.1–21% (vol.)
Concentration accuracy – CO2 < 1% (vol.)
Concentration accuracy – O2 < 0.5% (vol.)

14. Temperature control
ambient +3 °C up to 42 °C
Uniformity < 0.5 °C

15. Te-Cool cooling module
Temperature range : +18–42 °C
Cooling power : max 12 °C below ambient

16. Shaking
Linear, orbital, double-orbital;
variable amplitudes and frequencies"

主要功能及特色

"1、功能全面：紫外-可见光吸收光（Abs）、连续波长荧光强度（FI）、均相时间分辨荧光（HTRF）、荧光偏振（FP）、常规及快速荧光共振能量传递功能（FRET）、瞬时化学发光功能、延迟化学发光功能、发光共振能量转移（BRFT）、ALPHA检测系统 、超微量检测等、气体控制、自动开盖、湿度控制等动力学检测环境控制模块；
2、灵敏度高：荧光灵敏度、发光灵敏度、ALPHA等；
3、检测重复性好：波长准确性好、检测结果稳定、重复性佳；
4、检测灵活性好：荧光配置四光栅和滤光片融合光路，激发/发射可随意选择光栅或者滤光片；
5、配置环境控制模块：温控控制、湿度控制盒、气体控制模块、自动开盖模块，满足长时间动力学实验；"
"紫外-可见光吸收光（Abs）、连续波长荧光强度（FI）、均相时间分辨荧光（HTRF）、荧光偏振（FP）、常规及快速荧光共振能量传递功能（FRET）、瞬时化学发光功能、延迟化学发光功能、发光共振能量转移（BRFT）、ALPHA检测系统 、超微量检测等;
Absorbance – incl. UV/VIS spectra、Fluorescence top & bottom - incl. spectra、Time resolved fluorescence (TRF) – incl. spectra、Fluorescence Polarization (FP)、FRET、TR-FRETLuminescence – glow, flash, multicolor, spectra、AlphaScreen®, AlphaLISA® & AlphaPlex®、NanoQuant plate."

主要附件及配置

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